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3d printed oct imaging plates  (Formlabs Inc)

 
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    Structured Review

    Formlabs Inc 3d printed oct imaging plates
    Viability assessment pipeline with biological assay validation. a. Proposed workflow for obtaining <t>D-OCT</t> viability mapping for non-destructive imaging. b. MCF-7 human breast adenocarcinoma cells were seeded in low-adherence U-bottom plate. Spheroids formed were subjected to viability challenges. c. Spheroids were loaded onto <t>custom</t> <t>3D-printed</t> plate for sequential volumetric OCT scans. Wells of 6 spheroids were imaged over 12 mins to acquire volumes at up to 32 time points. d. Trypan blue exclusion assay on dissociated spheroids paired with automated cell counter produced absolute quantitative viability measurements.
    3d Printed Oct Imaging Plates, supplied by Formlabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3d printed oct imaging plates/product/Formlabs Inc
    Average 90 stars, based on 1 article reviews
    3d printed oct imaging plates - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Non-destructive viability assessment of cancer cell spheroids using dynamic optical coherence tomography with trypan blue validation"

    Article Title: Non-destructive viability assessment of cancer cell spheroids using dynamic optical coherence tomography with trypan blue validation

    Journal: Biomedical Optics Express

    doi: 10.1364/BOE.533339

    Viability assessment pipeline with biological assay validation. a. Proposed workflow for obtaining D-OCT viability mapping for non-destructive imaging. b. MCF-7 human breast adenocarcinoma cells were seeded in low-adherence U-bottom plate. Spheroids formed were subjected to viability challenges. c. Spheroids were loaded onto custom 3D-printed plate for sequential volumetric OCT scans. Wells of 6 spheroids were imaged over 12 mins to acquire volumes at up to 32 time points. d. Trypan blue exclusion assay on dissociated spheroids paired with automated cell counter produced absolute quantitative viability measurements.
    Figure Legend Snippet: Viability assessment pipeline with biological assay validation. a. Proposed workflow for obtaining D-OCT viability mapping for non-destructive imaging. b. MCF-7 human breast adenocarcinoma cells were seeded in low-adherence U-bottom plate. Spheroids formed were subjected to viability challenges. c. Spheroids were loaded onto custom 3D-printed plate for sequential volumetric OCT scans. Wells of 6 spheroids were imaged over 12 mins to acquire volumes at up to 32 time points. d. Trypan blue exclusion assay on dissociated spheroids paired with automated cell counter produced absolute quantitative viability measurements.

    Techniques Used: Biomarker Discovery, Imaging, Trypan Blue Exclusion Assay, Produced

    Normalisation techniques to decouple motion from intensity levels. a. Sample spheroids demonstrated results of 3 variance formulations on motion estimation. Relative deviation (Eq. ) exhibits similar patterns to intensity variation (Eq. ). Our regression-adjusted variance approach removed bias from low intensity near the bottom of spheroids. OCT images shown are illustrative examples and do not reflect the data distribution, which is better presented in the box plot. Colour bars were adjusted for optimal visual clarity. b. Median motion (Eq. ) distributions of 60 spheroids per viability insult group. MIV exhibits a large range, unable to discern between spheroid groups. Regression adjustment reduces spread and improves separation. Viability distribution obtained via trypan blue exclusion shows high correspondence with MAV distributions.
    Figure Legend Snippet: Normalisation techniques to decouple motion from intensity levels. a. Sample spheroids demonstrated results of 3 variance formulations on motion estimation. Relative deviation (Eq. ) exhibits similar patterns to intensity variation (Eq. ). Our regression-adjusted variance approach removed bias from low intensity near the bottom of spheroids. OCT images shown are illustrative examples and do not reflect the data distribution, which is better presented in the box plot. Colour bars were adjusted for optimal visual clarity. b. Median motion (Eq. ) distributions of 60 spheroids per viability insult group. MIV exhibits a large range, unable to discern between spheroid groups. Regression adjustment reduces spread and improves separation. Viability distribution obtained via trypan blue exclusion shows high correspondence with MAV distributions.

    Techniques Used:



    Similar Products

    3d printed oct imaging plates  (Formlabs Inc)
    90
    Formlabs Inc 3d printed oct imaging plates
    Viability assessment pipeline with biological assay validation. a. Proposed workflow for obtaining <t>D-OCT</t> viability mapping for non-destructive imaging. b. MCF-7 human breast adenocarcinoma cells were seeded in low-adherence U-bottom plate. Spheroids formed were subjected to viability challenges. c. Spheroids were loaded onto <t>custom</t> <t>3D-printed</t> plate for sequential volumetric OCT scans. Wells of 6 spheroids were imaged over 12 mins to acquire volumes at up to 32 time points. d. Trypan blue exclusion assay on dissociated spheroids paired with automated cell counter produced absolute quantitative viability measurements.
    3d Printed Oct Imaging Plates, supplied by Formlabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3d printed oct imaging plates/product/Formlabs Inc
    Average 90 stars, based on 1 article reviews
    3d printed oct imaging plates - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Viability assessment pipeline with biological assay validation. a. Proposed workflow for obtaining D-OCT viability mapping for non-destructive imaging. b. MCF-7 human breast adenocarcinoma cells were seeded in low-adherence U-bottom plate. Spheroids formed were subjected to viability challenges. c. Spheroids were loaded onto custom 3D-printed plate for sequential volumetric OCT scans. Wells of 6 spheroids were imaged over 12 mins to acquire volumes at up to 32 time points. d. Trypan blue exclusion assay on dissociated spheroids paired with automated cell counter produced absolute quantitative viability measurements.

    Journal: Biomedical Optics Express

    Article Title: Non-destructive viability assessment of cancer cell spheroids using dynamic optical coherence tomography with trypan blue validation

    doi: 10.1364/BOE.533339

    Figure Lengend Snippet: Viability assessment pipeline with biological assay validation. a. Proposed workflow for obtaining D-OCT viability mapping for non-destructive imaging. b. MCF-7 human breast adenocarcinoma cells were seeded in low-adherence U-bottom plate. Spheroids formed were subjected to viability challenges. c. Spheroids were loaded onto custom 3D-printed plate for sequential volumetric OCT scans. Wells of 6 spheroids were imaged over 12 mins to acquire volumes at up to 32 time points. d. Trypan blue exclusion assay on dissociated spheroids paired with automated cell counter produced absolute quantitative viability measurements.

    Article Snippet: The horizontal, vertical, and in-plane pixel dimensions were sampled at 256 × 256 × 256 px , resulting in a lateral pixel separation of 12.18 μ m and an axial separation was 6.996 μ m . Custom OCT imaging plates were 3D printed (Formlabs), with 3 × 3 × 0.5 mm well for spheroid imaging.

    Techniques: Biomarker Discovery, Imaging, Trypan Blue Exclusion Assay, Produced

    Normalisation techniques to decouple motion from intensity levels. a. Sample spheroids demonstrated results of 3 variance formulations on motion estimation. Relative deviation (Eq. ) exhibits similar patterns to intensity variation (Eq. ). Our regression-adjusted variance approach removed bias from low intensity near the bottom of spheroids. OCT images shown are illustrative examples and do not reflect the data distribution, which is better presented in the box plot. Colour bars were adjusted for optimal visual clarity. b. Median motion (Eq. ) distributions of 60 spheroids per viability insult group. MIV exhibits a large range, unable to discern between spheroid groups. Regression adjustment reduces spread and improves separation. Viability distribution obtained via trypan blue exclusion shows high correspondence with MAV distributions.

    Journal: Biomedical Optics Express

    Article Title: Non-destructive viability assessment of cancer cell spheroids using dynamic optical coherence tomography with trypan blue validation

    doi: 10.1364/BOE.533339

    Figure Lengend Snippet: Normalisation techniques to decouple motion from intensity levels. a. Sample spheroids demonstrated results of 3 variance formulations on motion estimation. Relative deviation (Eq. ) exhibits similar patterns to intensity variation (Eq. ). Our regression-adjusted variance approach removed bias from low intensity near the bottom of spheroids. OCT images shown are illustrative examples and do not reflect the data distribution, which is better presented in the box plot. Colour bars were adjusted for optimal visual clarity. b. Median motion (Eq. ) distributions of 60 spheroids per viability insult group. MIV exhibits a large range, unable to discern between spheroid groups. Regression adjustment reduces spread and improves separation. Viability distribution obtained via trypan blue exclusion shows high correspondence with MAV distributions.

    Article Snippet: The horizontal, vertical, and in-plane pixel dimensions were sampled at 256 × 256 × 256 px , resulting in a lateral pixel separation of 12.18 μ m and an axial separation was 6.996 μ m . Custom OCT imaging plates were 3D printed (Formlabs), with 3 × 3 × 0.5 mm well for spheroid imaging.

    Techniques: